• DETERMINATION OF MICROSCOPIC PARTICLE COUNT OF CRYPTOSPORIDIUM AND GIARDIA CYSTS USING MEMBRANE FILTERS

    Author(s):
    Lawrence K Wang (see profile) , Mu-Hao Sung Wang, Betty C Wu
    Editor(s):
    Yuriy I Pankivskyi
    Date:
    2022
    Group(s):
    Science, Technology, Engineering and Mathematics
    Subject(s):
    Ecology, Analytical biochemistry, Water--Purification, Cryptosporidium, Giardia, Membranes (Technology), Particle counting (Water treatment plants), Microscopy
    Item Type:
    Book chapter
    Tag(s):
    STEM, innovation, environmental biology
    Permanent URL:
    https://doi.org/10.17613/97wt-bb20
    Abstract:
    Wang, LK, Wang, MHS, and Wu, BC (2022). Determination of microscopic particle count of Cryptosporidium and Giardia cysts using membrane filters. In: “Environmental Science, Technology, Engineering and Mathematics (STEM)”, Wang, LK, Wang, MHS and Pankivskyi, YI (eds.), 2022(12C), STEM-VOL2022-NUM12C-DEC2022. 21 pages. December 2022, Lenox Institute Press, Massachusetts, USA. https://doi.org/10.17613/97wt-bb20 ..... ABSTRACT: Cryptosporidium is an intestinal coccidian protozoan parasite about 3.5 micrometers (um) in diameter. Giardia is the genus name for a group of single-celled, flagellated, pathogenic protozoans about 8 micrometer (um) in diameter. Both Cryptosporidium and Giardia are normally found in a variety of vertebrates, such as mammals, birds, and reptiles, and are responsible for waterborne disease outbreaks. At present, no practical means of rapid detection for Cryptosporidium and Giardia cysts can be employed. The APHA/AWWA/WEF Standard Methods' microscopic count or particle count of plankton is currently used for monitoring a water treatment plant's efficiency for removal of Cryptosporidium and Giardia cysts. The authors of the Lenox Institute of Water Technology (LIWT) introduce a simple and more accurate microscopic method as a substitute to verify removal of cyst-size (Cryptosporidium and Giardia cysts) contaminants by a water treatment plant. It should be noted, however, the rapid particle count method has been developed by LIWT using the 8-um member filter which was certified by Millipore Corporation for retaining and counting the 8-um Giardia cysts. A 3.54-um member filter should be used for retaining and counting both the Cryptosporidium and Giardia cysts. The difference between the two counts will be the number of Cryptosporidium.
    Notes:
    KEYWORDS: Water Treatment, Cryptosporidium, Giardia Cysts, Particle Count , Microscopic Count, Microscopic Particle Count, Environmental Biology
    Metadata:
    Published as:
    Book chapter    
    Status:
    Published
    Last Updated:
    1 year ago
    License:
    All Rights Reserved

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